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DEP4

Gene
DEP4
Protein
FAD-dependent monooxygenase DEP4
Organism
Alternaria brassicicola
Length
581 amino acids
Function
FAD-dependent monooxygenase; part of the gene cluster that mediates the biosynthesis of depudecin, a highly oxidized eleven-carbon linear polyketide that acts as a histone deacetylase (HDAC) inhibitor and makes a small contribution to pathogenesis (PubMed:19737099). The reducing polyketide synthase DEP5 is the central enzyme in depudecin biosynthesis by yielding the backbone polyketide chain (PubMed:19737099). The monooxygenases DEP2 and DEP4, as well as the uncharacterized protein DEP1, then act as tailoring enzymes to modify the intermediate polyketide chain into depudecin (PubMed:19737099).
Similarity
Belongs to the FAD-binding monooxygenase family.
Mass
66.051 kDa
Sequence
MESFDIVVVGAGWYGLMAARTYLELAPETNLLIVDDSSTVGGVWSKERIYPSLFAQISHPLFEYSFYPMPKDDISPDGFVSGKTIHNYLTSFTRDHNLLPRLRLNTRVERVRRGPSDAGGWVLDIKEGNPLLCHKLIYATGANSSPIIPTWPRQTFEQPVIHSLDLGKHQDYIAKSVETATVVGRSKSSYDAVYHLLCEGKRVNWVMRDGLSGPFSLYAPTFMGLWNIADHISTRFASSFSPCIMNTSGFCYNFLQRNAVGRTLTNVYWRAANYLSVSHAEYWRTPNAEKLRPRPYSDGVFWGSGGIGIATAPDFWKVFHRGNITIHSTEIDSLSHGDVVNLKNGYSLATDIVIHCTGFDKGYTTFDPELREELGLQYNSKSISRWSLLDAKAEQTVDELLPYLRTAPLQDMDPDASSRPEQGPNRHFRRLVVPELAARGDRSILFPGHIHSAFTPLAAELQALWGVSWMLGWRELPPQEEMELEAATFNAWTRKRYLEQGKKHSYFIYDYIPYIDTLMRDLGLNPYRKSSVFEEWFVRYKPSDYGTILDEYRDIRRLSLECLQGTGGFAERTNGHGVNMK

Gene
DEP4
Protein
FAD-dependent monooxygenase DEP4
Organism
Fusarium langsethiae
Length
580 amino acids
Function
Part of the gene cluster that mediates the biosynthesis of depudecin, a highly oxidized eleven-carbon linear polyketide that acts as a histone deacetylase (HDAC) inhibitor and makes a small contribution to pathogenesis (PubMed:19737099, PubMed:28460114). The reducing polyketide synthase DEP5 is the central enzyme in depudecin biosynthesis by yielding the backbone polyketide chain (PubMed:19737099). The monooxygenases DEP2 and DEP4, as well as the uncharacterized protein DEP1, then act as tailoring enzymes to modify the intermediate polyketide chain into depudecin (PubMed:19737099).
Similarity
Belongs to the FAD-binding monooxygenase family.
Mass
66.534 kDa
Sequence
MSQPLETFDVIVIGAGWYGLMAARTFLELAPDTNLLILDDGKTVGGVWSKERIYPSLFAQISHPLFEYSFYPMPEEDISPDGFVSGKTIQKYLEAFAKDHGLILHLRLETRVEKVKRGVNSNEWILEIKGDKPLACSKLIYATGANSSPIIPKWPREGFEKPVIHSLDLGRYQDYIANNVQKAVVVGRSKSSYDAVYHLLCAGKKVNWVMRDGQSGPFSLYAPTFMGLWNIADHISTRFASSFSPCIMSTDGFCYNFFQRSALGRVLTNMYWRTANYLSVSHAEYWRTENSEKLRPRPYSDGVFWGSGGIGIATAPDFWETFHRGDVTIHSTEIESVSHKDVTNLKNGYSIATDIIIHCTGFDKGYGAFDPQLRNELGLEYNTKEFSRWTMLDEKADQTVDRLLPYICDSPNQYGDSEASRSTGQGPNRHYRRLVVPELAARGDRSILFPGHIHSAFTPLAAELQALWGVSWMLGWRDLPSKEEMETEAANFNAWTRKRYLEQGRKHSYFIYDYIPYIDTLMKDLGLDPFRKSNVFEEWFVRYKPSDYKTILEEYRSVRRHEREMERSGEESVARMKRCD