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DAD1

Gene
DAD1
Protein
Phospholipase A(1) DAD1, chloroplastic
Organism
Arabidopsis thaliana
Length
447 amino acids
Function
Sn-1-specific phospholipase that releases free fatty acids from phospholipids. Low activity on galactolipids and triacylglycerols. Catalyzes the initial step of jasmonic acid biosynthesis. Not essential for jasmonate biosynthesis after wounding or upon pathogen infection.
Similarity
Belongs to the AB hydrolase superfamily. Lipase family.
Mass
49.96 kDa
Sequence
MRFSLSPVRPHSVVVPSLPKQDVVSYISGTTSNRQCRCVLTLPSPSVSTSRPPVLPKPETWESLLLNHDQIPGEFSPTGSSIPVKLGRRWMEYQGLQNWDGLLDPLDDNLRREILRYGQFVESAYQAFDFDPSSPTYGTCRFPRSTLLERSGLPNSGYRLTKNLRATSGINLPRWIEKAPSWMATQSSWIGYVAVCQDKEEISRLGRRDVVISFRGTATCLEWLENLRATLTHLPNGPTGANLNGSNSGPMVESGFLSLYTSGVHSLRDMVREEIARLLQSYGDEPLSVTITGHSLGAAIATLAAYDIKTTFKRAPMVTVISFGGPRVGNRCFRKLLEKQGTKVLRIVNSDDVITKVPGVVLENREQDNVKMTASIMPSWIQRRVEETPWVYAEIGKELRLSSRDSPHLSSINVATCHELKTYLHLVDGFVSSTCPFRETARRVLHR

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Gallus gallus
Length
123 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.926 kDa
Sequence
MSGTAGSGVGAAGSVGSVVRRFLAEYGSGTSSRLKVLDAYLLYVMLTGALQFGYCLGVGTFPFNSFLSGFISAVGSFILGVCLRIQINPQNKGEFQGISPERAFADFLFANTILHLVVINFVG

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Canis lupus familiaris
Length
119 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
13.11 kDa
Sequence
MSASVASVISRFLEEYLSSTPQRLKLLDAYLLYILLTGALQFGYCLLVGTFPFNSFLSGFISCVGSFILAGNGSLRNRSNNVFTLVRCFSSLVTLFYSRSPPREVPRGACIALFCERGN

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Malus domestica
Length
119 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.84 kDa
Sequence
MGKASHSSTAQDAVALFDSLRSAYSATPTTLKIIDLYIGFAVSTALIQVVYMAIVGSFPFNSFLSGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHMVIMNFLG

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Pisum sativum
Length
117 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.88 kDa
Sequence
MAKTSSTTKDAQDLFHAIWSAYSATPTNLKIIDLYVVFAVFTALLQDVYMALVGPFPFNSFLSGVLSCVGTAVLAVCLRIQVNKENKEFKDLGPERAFADFVLCNLVLHLVIMNFLG

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Solanum lycopersicum
Length
116 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.671 kDa
Sequence
MAKSSATKDAQALFHSLRSAYAATPTNLKIIDLYVIFAISTALIQVVYMAIVGSFPFNSFLSGVLSCIGTAVLAVCLRIQVNKENKEFKDLPPERAFADFVLCNLVLHLVIMNFLG

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Arabidopsis thaliana
Length
115 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.695 kDa
Sequence
MVKSTSKDAQDLFRSLRSAYSATPTNLKIIDLYVVFAVFTALIQVVYMALVGSFPFNSFLSGVLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVIINFLG

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Betula pendula
Length
115 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.575 kDa
Sequence
MARSTSKDAQALIQSLRSAYAATPSNLKIIDLYVVFAVFTALIQVVYMAIVGSFPFNAFLSGLLSCIGTAVLAVCLRIQVNKENKEFKDLAPERAFADFVLCNLVLHLVIMNFLG

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Citrus unshiu
Length
115 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.547 kDa
Sequence
MARSTGKDAQALFHSLRSAYAATPTTLKIIDLYVGFAVFTALIQVVYMAIVGSFPFNSFLSGVLSCVGTAVLAVCLRIQVNKDNKEFKDLPPERAFADFVLCNLVLHLVIMNFLG

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Picea mariana
Length
115 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.514 kDa
Sequence
MGTSTAKEAHALIASLRSAYSATPTKLKIIDLYVVYAILTAVVQVVYMAIVGSFPFNAFLSGVLSCTGTAVLAVCLRMQVNKENREFKDLPPERAFADFVLCNLVLHLVIMNFLG

Gene
DAD1
Protein
DASH complex subunit DAD1
Organism
Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)
Length
114 amino acids
Function
Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore (By similarity).
Similarity
Belongs to the DASH complex DAD1 family.
Mass
12.043 kDa
Sequence
MIMSKDIATTPELSEPDKYFVEQRDLLLQEITSTLDSILNNLNGLNISLENSIAVGKEFESVSELWKVFYDGLANGAAPGVAAANPSSQDLPTEPVAAHQNAAAGNSDAPAPSQ

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Hordeum vulgare
Length
114 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.347 kDa
Sequence
MPKAAGDAKLLIQSLNKAYAATPTNLKIIDLYVVFAVVTALLQVVYMGIVGSFPFNSFLSGVLSCIGTAVLAVCHRIQVNKDNKEFKDLAPERAFADFVLCSLVLHLVIMNFLG

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Oryza sativa subsp. indica
Length
114 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.379 kDa
Sequence
MPRATSDAKLLIQSLGKAYAATPTNLKIIDLYVVFAVATALIQVVYMGIVGSFPFNSFLSGVLSCIGTAVLAVCLRIQVNKDNKEFKDLPPERAFADFVLCNLVLHLVIMNFLG

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Oryza sativa subsp. japonica
Length
114 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.379 kDa
Sequence
MPRATSDAKLLIQSLGKAYAATPTNLKIIDLYVVFAVATALIQVVYMGIVGSFPFNSFLSGVLSCIGTAVLAVCLRIQVNKDNKEFKDLPPERAFADFVLCNLVLHLVIMNFLG

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Araneus ventricosus
Length
113 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.601 kDa
Sequence
MGSSAFEVLTFFLKDYKANTPQKLKIIDAYLLYILLTGINQFLYCCLVGTFPFNSFLSGFISCVASFVLGVCLRLQVNPQNSSNFCGIPPERAFADFIFAHVVLHLVVMNFIG

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Bos taurus
Length
113 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.495 kDa
Sequence
MSASVLSVISRFLEEYLSATPQRLKLLDAYLLYILLTGALQFGYCLLVGTFPFNSFLSGFISCVGSFILAVCLRIQINPQNKADFQGISPERAFADFLFASTILHLVVMNFVG

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Homo sapiens
Length
113 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation (PubMed:22467853). N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). Required for the assembly of both SST3A- and SS3B-containing OST complexes. Loss of the DAD1 protein triggers apoptosis (PubMed:22467853).
Similarity
Belongs to the DAD/OST2 family.
Mass
12.497 kDa
Sequence
MSASVVSVISRFLEEYLSSTPQRLKLLDAYLLYILLTGALQFGYCLLVGTFPFNSFLSGFISCVGSFILAVCLRIQINPQNKADFQGISPERAFADFLFASTILHLVVMNFVG

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Mesocricetus auratus
Length
113 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.497 kDa
Sequence
MSASVVSVISRFLEEYLSSTPQRLKLLDAYLLYILLTGALQFGYCLLVGTFPFNSFLSGFISCVGSFILAVCLRIQINPQNKADFQGISPERAFADFLFASTILHLVVMNFVG

Gene
Dad1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Mus musculus
Length
113 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.497 kDa
Sequence
MSASVVSVISRFLEEYLSSTPQRLKLLDAYLLYILLTGALQFGYCLLVGTFPFNSFLSGFISCVGSFILAVCLRIQINPQNKADFQGISPERAFADFLFASTILHLVVMNFVG

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Sus scrofa
Length
113 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.511 kDa
Sequence
MSASVLSVISRFLEEYLSSTPQRLKLLDAYLLYILLTGALQFGYCLLVGTFPFNSFLSGFISCVGSFILAVCLRIQINPQNKADFQGISPERAFADFLFASTILHLVVMNFVG

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Pongo abelii
Length
113 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.55 kDa
Sequence
MSASVVSVISRFLEEYLSSTPQRLKLLDAYLLYILLTGALQFGYCLLVGTFPFNSFLSGFISCVGSFILAVRLRIQINPQNKADFQGISPERAFADFLFASTILHLVVMNFVG

Gene
Dad1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Rattus norvegicus
Length
113 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.497 kDa
Sequence
MSASVVSVISRFLEEYLSSTPQRLKLLDAYLLYILLTGALQFGYCLLVGTFPFNSFLSGFISCVGSFILAVCLRIQINPQNKADFQGISPERAFADFLFASTILHLVVMNFVG

Gene
dad1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit dad1
Organism
Xenopus laevis
Length
113 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
12.596 kDa
Sequence
MSVSVFSVVSRFLDEYVSSTPQRLKLLDAYLLYILLTGALQFLYCLLVGTFPFNSFLSGFISSVGSFILAVCLRIQINPQNKSDFQGISPERAFADFLFANTILHLVVVNFIG

Gene
Dad1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Drosophila melanogaster
Length
112 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity (By similarity). Probably as part of the N-glycosylation pathway, plays a role in the regulation of tissue growth and apoptosis (PubMed:27693235).
Similarity
Belongs to the DAD/OST2 family.
Mass
12.526 kDa
Sequence
MVELSSVISKFYNDYVQNTPKKLKLVDIYLGYILLTGIIQFVYCCLVGTFPFNSFLSGFISTVSCFVLAVCLRLQANPQNKSVFAGISPERGFADFIFAHVILHLVVMNFIG

Gene
DAD1
Protein
DASH complex subunit DAD1
Organism
Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / JCM 1990 / NBRC 0083 / IGC 2968)
Length
104 amino acids
Function
Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore (By similarity).
Similarity
Belongs to the DASH complex DAD1 family.
Mass
11.865 kDa
Sequence
MSESSTYFEKQRDMLIQEIGVSIESVVYNLDILNRSLNGSISVGKEFDNVGRLWSHFYDGLNQLKERNNQEETNKNDQGDANDDDSSSDEHGSSDEEIDQDQEN

Gene
DAD1
Protein
DASH complex subunit DAD1
Organism
Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)
Length
95 amino acids
Function
Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore (By similarity).
Similarity
Belongs to the DASH complex DAD1 family.
Mass
10.779 kDa
Sequence
MTEEVQQLSTGDKYFIEQRSLILQEINESMDAILNQLNGLNITLENSIAVGKEFENVSQIWKLFYNGLESEEQNMETVGVADSEHITEDVNPEGK

Gene
DAD1
Protein
DASH complex subunit DAD1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Length
94 amino acids
Function
Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. The DASH ring complex may both stabilize microtubules during chromosome attachment in anaphase A, and allow the chromosome to remain attached to the depolymerizing microtubule in anaphase B. Microtubule depolymerization proceeds by protofilament splaying and induces the kinetochore-attached ring to slide longitudinally, thereby helping to transduce depolymerization energy into pulling forces to disjoin chromatids.
Similarity
Belongs to the DASH complex DAD1 family.
Mass
10.516 kDa
Sequence
MMASTSNDEEKLISTTDKYFIEQRNIVLQEINETMNSILNGLNGLNISLESSIAVGREFQSVSDLWKTLYDGLESLSDEAPIDEQPTLSQSKTK

Gene
dad1
Protein
DASH complex subunit dad1
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843)
Length
90 amino acids
Function
Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore. Mediates interaction between the DASH and sim4 complexes leading to loading of DASH onto the kinetochore.
Similarity
Belongs to the DASH complex DAD1 family.
Mass
10.523 kDa
Sequence
MSSENMDITENIQNEQNKDFDDIDFERRRRLLTLQISKSMNEVVNLMSALNKNLESINGVGKEFENVASLWKEFQNSVLQKKDREMLDAP

Gene
DAD1
Protein
DASH complex subunit DAD1
Organism
Yarrowia lipolytica (strain CLIB 122 / E 150)
Length
88 amino acids
Function
Component of the DASH complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The DASH complex mediates the formation and maintenance of bipolar kinetochore-microtubule attachments by forming closed rings around spindle microtubules and establishing interactions with proteins from the central kinetochore (By similarity).
Similarity
Belongs to the DASH complex DAD1 family.
Mass
10.306 kDa
Sequence
MSRVSMFPTEDKRSYFERQRDQYIKNISASMEDVLNNLNALNRSLEHTVGLSQKSEELAEMWSHFYNTVNEDLVKTEDGDIEVDTTKE

Gene
DAD1
Protein
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
Organism
Zea mays
Length
79 amino acids
Function
Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity.
Similarity
Belongs to the DAD/OST2 family.
Mass
8.545 kDa
Fragment
single
Sequence
AVATALIQVAYMGLVGSFPFNSFLSGVLSCIGTAVLAVCLRIQVNKDNKEFKDLPPERAFADFVLCNLVLHLVIMNFLG