Protein
Eukaryotic translation initiation factor 5B
Organism
Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)
Function
Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.
Similarity
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. IF-2 subfamily.
Sequence
MPPKGKKNRKNDDWDADLGESIAPVNPAAAAPAAAEAPAAAEKEEEAPVGGLMALMRKNKEKRKKKGLSEDWLDGETPEGQAPAAAPEPDLSAKQAEEANLEDEWALPDKKGKKGGKQQQNKKQQQEEKKKDDEEVPEIRVLTKAEKERLKKEREKQRKKEQAAKKKAAGPAQPQKAEPAKPAEEEKKPEEPAPAPAPAPEPAAGGSKKKIPAHLRLIQKQQEELRRRQEEEQRRLEEERRRIEEEERRAEEERKRKEEEKARKKQKEKEKIEQLKREGKYLTKAQREEKARNQRMLEQMRAAGIKIAALEEKNKEEGAAEGEKEKKEKKKPEKKRRPNKVDEQKALEEAAERARQQAEAAAREAEEKARLEREKAEAEEKAKQAAAEESVDEDWEAAAESDKEDVPDSWDAAASDEEKEEEEEEEEEEEKPAPKKTEKKPEPKTEEKKKAEPKKPEAKKEEVKKPEPKKEEPKKAEANGKPATTTLPTRSKEEEKPKAAEAEKKEVAARPKKPVVNKDNLRSPICCILGHVDTGKTKLLDKIRQTNVQEGEAGGITQQIGATYFPVEAIKQKTAVVNKDGKFEFKVPGLLIIDTPGHESFSNLRSRGSSLCNIAILVVDIMHGLEPQTIESLRLLRERKTPFVVALNKIDRLYGWKKIENNGFRESFALQNKAVQNEFRNRLDQVKLQFAEQGFNSELFYENKNFARYVSLVPTSAHTGEGIPDMLKLIVQLCQERMASSLMYLSELQATVLEVKAIEGFGVTIDVILSNGILREGDRIVLCGLEGPIKTNIRALLTPAPMRELRIKGQYIHHKEVKAAQGVKISAPGLEGAIAGSRLLVVGPDDDEEELEEEVESDLQSLFSRVEKTGKGVSVQASTLGSLEALLDFLKDCKIPVANVGIGPVYKRDVMQCGIMLEKAPDYAVMLCFDVKVDKEAQQYADENGIKIFTADIIYHLFDQFTKHMQEQLEKKKEESKMLAVFPCVLNPVAVFNKTNPIVVGVDVVDGQLKLNTPIAAVKMNPTTGQKEIISLGRVTGIERDHKPLQVCKKGQPAVAIKIEMGGHQPAYGRHLDEKDVLYSHISRASIDVLKQFYRDVVTTDEWQLIIKLKSVFDVQ