BRO1

Gene

bro1

Protein

Vacuolar protein-sorting protein bro1

Organism

Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)

Length

976 amino acids

Function

Involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles.

Similarity

Belongs to the BRO1 family.

Mass

108.306 kDa

Sequence

MVQSPMLSCPLKQTNEIDWIRPLKDYIRQSYGEDPERYNQECATLNRLRQDMRGAGKDSATGRDLLYRYYGQLELLDLRFPVDENHIKISFTWYDAFTHKPTSQYSLAYEKASIIFNISAVLSCHAANQNRAEESGLKTAYHSFQASAGMFTYINENFLHAPSTDLNRETVKTLINITLAQAQEVFLEKQVTDQKKAGFLAKLASQAAYLYSQAAEGIQEYAKGVFDKSWTIVVQAKAAHMASVASYYQALADSESNSHGVAIARLQLADKNSTAAMGWANLVDTIKYHQANVQVKLATFVKDNDFIYHQPVPNEAGLSAVAKLPAAKAIPVSELYQGQDIQRIIGPDIFQKLVPMSVTETASLYDEEKAKLIRAETEKVETADSEMAASLDYLKLPGSLNILKGGMDQEMTVDDEFRQWCQELAGHQSFAKAFDTLQDRKGEILSQLDRCSKQLDLEESVCEKMRSKYGADWSQQPSARLNSTLRSDIRTYRDTINEASASDSQLLATFRQYETDFDEMRSAGETNEADVLFQRAMIKAGSKHGKGRNGVGSPYASTQEGSLLDDVYDEGSLSVAEQIARVESILKKLNLVKRERSQVLKDLKEKVHNDDISNVLILNKKSIAGQESQLFETELEKFRPHQNRLLQANHKQAALMKELTKVYGDLLQDKRVRSEQSKYEAITRQRNTVMARYKKIYDAFNGLLSGIAQAQTFYTEMVETVESLKKNVETFINNRRSEGAQLLGQIEREKAGTATDQEDREREKLRQLMERLSTEPKPTSTPLPSTAPSKAKSPPPPVKAPGYPGPGIASPQMSPHFAPGVAGQQHGIPLSHSPAPYGQYVAPPSGVSYMQGQPFQQGAAAPLSEGYNPMAYPAPTSISPPPSQQYYSSTPAPYSGYSNPAPPNAPSQFMPQGYVPPPPPPRPQQPSYPPSTGPYPSGPGGYAQSRPYGTSQHHKTPSQSQSSSSTDPWAGLNAWK

Protein

Vacuolar protein-sorting protein bro1: bro1

Gene

BRO1

Protein

Vacuolar protein-sorting protein BRO1

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876)

Length

945 amino acids

Function

Involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles.

Similarity

Belongs to the BRO1 family.

Mass

108.853 kDa

Sequence

MKTHLLVVPSKKTEEVNWVKPLNNYLLSIYGNTLQYQDDINSLNKLRQDIRGVNADDTGLKLYYSYYSKLELIDLRIPFHDLNKSKKLQFEWFDSFSSLPYTQNSLAFEKANVLYNIGAILSRFAQFKYNESQQLNGPEGETAFKQSISMLQQSSGIHQFINKNFLHAPSQDLAQSTIKFLSKLMMAQSQEIFTLKVISGDLNQSKNSLIAKLCKSTSVLYEDCYNMINKNKKEGFKITNESYDGNDYEDDLDDDDCDDLEGFLERPEDTEDSDNRYVAAELDSSWISIIYFKYEYYKSLAYYFNGLHLDANRKYGDAIAYLTKSQEVLNGVHSSTLKQVSKGSGDVYELLDNYKYQKDAVTIRLNDLNKDNDLIYHDIVPNLVTLPEIKPMDSTKIIKLGDNAMFHEINEKNYNNFLVNVVPVNIHELSSYYSEEKSQYLRNEIDHFEVSNEEVSSVLEYLKLPKALVSIKESLRGTSSKGNTRNIDELLSQSVIDKVNEISSQYSNDIQRKNTIENSRKEIYQLIKFLTENNCQDDAIQLKKILYDASVSDTKLFALVDETYHQTLGKGIQSTEFKSLFKTVDNKSSNQDSEISLLDIIDDDDDDNTNQDKQIKNIEDYLNDLNNIKQAKFKLIEKLKKEIHNDDISDILVLNMKLKSTNEIKSIIFPEELKKFNPFTNELDKLITNEKSIIEQLKSRWNQLISNPQIKNLQTETNSKNRLIISQSEKIDQFYGNWLKYHNGLIAGEKFYNQLLDQFKNFRNNIDNQLNQGMRSLNIGQQFPTQPMLNRTQSSGLQSNYTGQSSISHSGSLSGQPSGGYNQPQPQQQQLQQQQQQQQQAQFSGAYNQQYSQPHGGYTQFQGYGQGQGQSQTGYQRQPPPPPPQLPPKQSMGSYNFATTLQQQQLPPQQQQQPQQQRTPQNESNLIYDNPSTYNPNMYNFFSSK

Protein

Vacuolar protein-sorting protein BRO1: BRO1

Gene

BRO1

Protein

Vacuolar protein-sorting protein BRO1

Organism

Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)

Length

888 amino acids

Function

Involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles.

Similarity

Belongs to the BRO1 family.

Mass

101.13 kDa

Sequence

MKPSVIGLKCKDTDKVDWKRGLSSYLKRIYGSRQWKEFYDEQLCVEMDHVRNNANGELGAVTLVEQNYKYYAYLEQLYLRLGNNIGQFKLEFTWYDAEYGLVSSPTKHTQKTLVFEKSCTLYNLGVALTEVANEKINEDFKTAMVHMAKAMECFRYLSENFFNSPSADLQTENTKFLSDLSHAEAQEMFLINAINNGTSEKQASLISKLAYSGSNLYENCWEFLRTEEGGLTPYGEARWNSIVSGKHHFFRSLAAYYNALALEQNNKYGEAIAFLKLATQCLSSSLPYKYALNDNFDFDGFGETIKDKTKQLIKDNDYIFHDSIPQSVSLSSIKALDAIKAPKWEEQLKPFMEAIAHKCEKLYRGIVPMEVFEKESIYSEKKASMLRQCINDSETADMEYSSFIEFTHLPNLLSDLKRRYKSQNFSGTTDPQGDMMRDQIQSWIKSISQSKYKDPDEQLKLISSKKQEILTLLAGLPSEQKENVVKLKMALVEAAASDEKLFSLVQPYAAQLRLLKQPDELWKIFNMFSIDESNKQSLLDIDDSKNQEILAKISDIEQMAEDLRLLKEERGRTLKELKSQTNDDDITNTLLVNSKAESEELEVIFKKELDKFKPLTTRIEATIFKQESVVNEVKNELDNVFSLSGLENKTSEEEEKQKKRKEFFMQIEEAATKFLIFNNDLPKGLEFYDSLLKMSKDLAVSVKVQNNASGSDNNSNNGYVSGNVIPPSLPPQPRNVGSSIDSQFQSMNLGSVPTPQRFPQPPAPNSRPIVSMENYTSQFSVPPAHGDLPPAYNQVPLVPTRNYDQPHGSGNYPGNVSSQHPVSSSPIPGAYDQVPMVPPKQPPAEGRSQISRQEQMEREERELQRDPTAFYKKSSVFDESLYSRYSGK

Protein

Vacuolar protein-sorting protein BRO1: BRO1

Gene

BRO1

Protein

Vacuolar-sorting protein BRO1

Organism

Arabidopsis thaliana

Length

846 amino acids

Function

Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen (Probable). Associates with FREE1 and ELC to perform function in the ESCRT-I complex. Binds ubiquitin in vitro (PubMed:26902184). Plays a role in the biogenesis of vacuole and multivesicular bodies (MVBs) (PubMed:26342016, PubMed:26324913). Required for the endosomal location of AMSH3 (PubMed:26324913). Mediates high-affinity phosphate transporter trafficking to maintain phosphate homeostasis. Regulates vacuolar degradation of PHT1-1 (PubMed:26342016).

Mass

95.209 kDa

Sequence

MASSSLSNLMLAIHEKKTSSVDLYRPLRNYVTFTYSEREAQLIDDDLETLKQLRSDIERVSDPSPAARRDLLISYYKVLCLVETRFPISPDKDHVNAVSFVWYDAFKQKHKATQQNIHLEKAAVLFNLGASYSQIGLGHDRTTVDGRRQASHAFMAAAGAFAHLRDNESIKATIGPSTTVDVSVECVGMLERLMVAQAQECVFENTIAKGSTPGVSAKIARQVGIFYEEALSALIISPLKDHFDKGWISHVQLKAALFYGEACFRYGKELHEKEEIAEEIARLRSGASRLAEAKKSSRGAPAQLIEAMNTLESSINGNLDRAVKENDRVYLMRVPSPSSLSPLPAFSMVKPMNMTDILDASKEKMFSILVPDSSAKALSRYTEMVDDVIRTQAERLQQASELTRVRLKEMDLPDSILAVDGNSALPVDLKEDVEAVQISGGPAGLEAELQQLRDLKRVNQELLVHTEELLQKEATEDSQFRSQFGTRWTRPQSSTLTKNLQDRLNRFAANLKQAGESDVKIERSVRDNSALMSILDRRPIESAVPTLARPIMSLDATEDAIVGTLKQSLRQLENLGAQRAGLEDMLKEMKRKDDILPKLMTITGSYEDMFRKEISKYDHICEDISQNIEVQEQLLMQIQAQNEEFSTIFNLEDYKASKEKCYKQIQAAIMKYREIKENINEGLKFYVTLQDAITNVKQQCSDFVMTRSIQCRDMIEDVQRQMSGLSFQDHRSSGPYPSVHQPTASSPPPPPETQNPSHPHPHAPYYRPPEQMSRPGYSIPPYGPPPPYHTPHGQAPQPYPPQAQQQPHPSWQQGSYYDPQGQQPRPPYPGQSPYQPPHQGGGYYRQ

Protein

Vacuolar-sorting protein BRO1: BRO1

Gene

BRO1

Protein

Vacuolar protein-sorting protein BRO1

Organism

Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)

Length

834 amino acids

Function

Involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles.

Similarity

Belongs to the BRO1 family.

Mass

94.362 kDa

Sequence

MKTALIGLKLKDTEPLDWSKALASYLKRNYGQHQWSQFYDDKKSAELDQLRISANSDLGLEALLEQNLRYYGFLEQLYLRLGSQSVQLKLDYTWYDAEHGMNGGQKYKQHTLVFEKSSTLFNIGVLLAQLAGEQLRASYKEAIPQLARAVACFEYMSETFLNSPSVDLQAENTGFLAALLHAEAQELFLLNVINGGDAARRASLLCRLSYTCARFYRKCVEFYKADGADEAAAAVPYGEAKWRDVVNMKMHLYEGVAAYNSALVLEQAGKIGHAVGLLEKAAASMATANTHRKALQGTAYLRDEVDLDGISSVVNEKLKTTAKDNDYIYHDHVPSDISLDSTKLMDAIKVPEFDAVVGPYLEAATETCNDLFRGIVPMAVYERESMYTEEKTQLLRREVDAVEQADWEYQSFVDFTNLPKLLDDLERRFLDTSSSSEDAEVVHMREQLQSWADAVRQSSFNDIEQQSKNILRTRNDIMALLSSVPPENKEDVVKIKSSLLQASQSDEKLFSQVKPYVNEIQLLNNPTLLWNNFNTFTTSQPTPDLLDLDHSKADEVLAKIRQIRQLYENLKLLKEERTSIMKDLKDLVNQDDITKQLILNNNKSDSQIKTLFQEELEKFRPFGSRIEATVFKQGNTIKDIKIGLDTIFKLADVQEKTSAQKQAASQRKEFFSKLQKAATAFKLFETDLPKGLSFYDLLYKMTKEMHDLSVTKRTTDSDLASNMAGLNISSSKETAKYNPQPLTAPPLAPRTYMPPPQAYNRNLDIRNANTYGNNLNAGSLGQRPRLPQKIPTGISGLETQNDPKLSEEESIRNPTNFYNTPSVFNESMYSKFSH

Protein

Vacuolar protein-sorting protein BRO1: BRO1