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AOL_s00215g282

Gene
AOL_s00215g282
Protein
Cytochrome P450 monooxygenase AOL_s00215g282
Organism
Arthrobotrys oligospora (strain ATCC 24927 / CBS 115.81 / DSM 1491)
Length
537 amino acids
Function
Cytochrome P450 monooxygenase; part of the gene cluster that mediates the biosynthesis of arthrosporols, metabolites that possess a novel hybrid carbon skeleton consisting of a polyketide-derived epoxycyclohexenol combined with a terpenoid-derived monocyclic sesquiterpenol substructure. Arthrosporols B and C are two dehydration derivatives of arthrosporol A. All of the arthrosporols shared the same epoxycyclohexenol moiety, and the difference among them only occurred in the monocyclic sesquiterpenol substructure (PubMed:26422178, PubMed:27723963). The pathway begins with the biosynthesis of 6-methylsalicylic acid (6-MSA), the first precursor of the polyketide-derived epoxycyclohexenol in arthrosporols, by the polyketide synthase (PKS) AOL_s00215g283 via condensation of 1 acetate and 3 malonate units. AOL_s00215g283 performs a series of programmed reactions including Claisen condensation, dehydration, reduction, and cyclization to yield 6-MSA (PubMed:26422178). The 6-methylsalicylic acid decarboxylase AOL_s00215g281 then catalyzes the decarboxylation of 6-methylsalicylic acid to yield m-cresol (PubMed:27723963). The arthrosporols precursor m-cresol might be a versatile precursor for other pathways (PubMed:27723963). The cytochrome P450 monooxygenase AOL_s00215g282 then acts as a m-cresol hydrolase that converts m-cresol to arthrosporols, in combination with other arthrosporols cluster's enzymes such as the cytochrome P450 AOL_s00215g280 (PubMed:27723963, PubMed:28475838).
Similarity
Belongs to the cytochrome P450 family.
Mass
61.549 kDa
Sequence
MIPINISPATVVLCGSIVTVSIAYVIFVVGHRRKGFPPGPPTLPVIGNIHQLPTTGAHFKFTEWAQKYGGIYSLKLGTGTALVITDREIVKKLMDKKSSITSDRPPSYVSYDLITKGDHLLVMHYNETWRKIRKYMHQFFRESECEKKHITLQDAEATQMMLEFLETPDNHNLHTKRFSNSIISTLLFGLRTPTYDSPHAKHLYDIMERWSAVMEPGNTPPVDIYPIFKYIPESLFGNWISRASGVGRDMERLYANMISQFKIRAEDPKISRTHESFFDILLREQEKTREFTDHEIAFIGGVQLEGGSDTSASILTAFVQAMVNWPEVQRVAQEEIDRVIGEDRSPTWNDFAALPYINAIMKETHRWRPVTPLSFPHCLTKDEYLDGFLLPKGATIILNVWGIQNDESVYPRPEEFDPSRFKDHKLLAPEYAVSDWKLRDHYSFGVGRRICPGIHLAERNVWLGVAKLLWAFKFEKATGADGQPIEMNVDPKTGYTEGFLHCALPFKCKVTVRSEERRKTILKEFELAKQVFNEYTG